DNA identification of urban Tanytarsini chironomids (Diptera:Chironomidae)

摘要: AbstractDNA barcoding is becoming widely used to provide species identifications in a variety of invertebrate taxa, but there has been little application so far environmental monitoring. Here we make this connection using group aquatic macroinvertebrates, chironomids the tribe Tanytarsini. Tanytarsini larvae commonly collected biological surveys can be difficult identify because high intraspecific variability and not all are linked described adult life stages. We examined whether could reliably identified with polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) mitochondrial cytochrome oxidase I (COI) gene. were from lentic environments consisted 3 common genera (Cladotanytarsus, Paratanytarsus, Tanytarsus). COI PCR-RFLP profiles discriminate larvae, including morphologically similar sequences ...