Response of Syngonium podophyllum L. "White Butterfly" shoot cultures to alternative media additives and gelling agents, and flow cytometric analysis of regenerants

摘要: Abstract. Teixeira da Silva JA. 2015. Response of Syngonium podophyllum L. ‘White Butterfly’ shoot cultures to alternative media additives and gelling agents, flow cytometric analysis regenerants. Nusantara Bioscience 7: 26-32. (arrowhead vine) is a popular leafy indoor pot plant whose tissue culture has been established, primarily through in vitro culture, but several interesting aspects have not yet explored. In this study, cv. was used investigate the response formation agents additives. Gellan gum (Gelrite®) at 2 g/L resulted greater leaf production, plantlet fresh weight higher chlorophyll content (SPAD value) than all other tested, including agar, Bacto phytagel, oatmeal potato dextrose barley starch corn starch, when on basal Hyponex® (NPK = 6.5: 6: 19; 3 g/L) medium. Several liquid medium tested (low full-fat milk, Coca-Cola®, coffee, Japanese green, Oolong, Darjeeling teas) negatively impacted growth, stunted roots decreased leaves. Plant growth with refined sucrose or table sugar responded similarly. Poor observed crude extract from high rebaudioside-containing stevia (Stevia rebaudiana Bertoni) line - an artificial sweetener used. Leaf control did show any endopolyploidy low levels (8C) were detected some treatments.