Cryoprotective properties of completely synthetic polyampholytes via reversible addition-fragmentation chain transfer (RAFT) polymerization and the effects of hydrophobicity.
作者： Robin Rajan , Minkle Jain , Kazuaki Matsumura
DOI: 10.1080/09205063.2013.801703
关键词:
摘要: A completely synthetic polyampholyte cryoprotectant was developed with cationic and anionic monomers by reversible addition-fragmentation chain transfer polymerization. The neutralized random polyampholyte, which had an equal composition ratio of monomers, showed high cryoprotective properties in mammalian cells. Introduction a small amount hydrophobic monomer enhanced cell viability after cryopreservation, indicating the importance hydrophobicity. Leakage experiments confirmed that these polyampholytes protected membrane during cryopreservation. Due to low cytotoxicity, this has potential replace convention agent dimethyl sulfoxide. present study is first show we can design polymeric will protect freezing using appropriate polymerization techniques.
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