Cell encapsulation and cryostorage in PVA–gelatin cryogels: incorporation of carboxylated ε-poly-L-lysine as cryoprotectant

摘要: It is desirable to produce cryopreservable cell-laden tissue-engineering scaffolds whose final properties can be adjusted during the thawing process immediately prior use. Polyvinyl alcohol (PVA)-based solutions provide platforms in which cryoprotected cell suspensions turned into a ready-to-use, scaffold by of cryogelation. In this study, such PVA system, with DMSO as cryoprotectant, was successfully developed. Vascular smooth muscle (vSMC)-encapsulated cryogels were investigated under conditions cyclic strain and co-culture vascular endothelial cells mimic environment these experience vivo setting. view cytotoxicity imposes respect production procedure, carboxylated poly-L-lysine (COOH-PLL) substituted non-cytotoxic cryoprotectant allow longer, slower periods generate more stable cryogels. Encapsulated vSMC responded 10% increased alignment proliferation. Cells stored frozen for 1 month without loss viability compared immediate thawing. SMC-encapsulated also supported functional co-culture. Substitution COOH-PLL place resulted significant increase encapsulated range periods. We conclude that incorporation cryogelation preserved functionality while retaining fundamental cryogel physical properties, thereby making it promising platform scaffolds, particularly tissue engineering, or preservation within microgels.