Chitosan-Based Nanocoatings for Hypothermic Storage of Living Cells

摘要: The formation of ultrathin chitosan-based nanocoating on HL-60 model cells and their protective function in hypothermic storage are presented. encapsulated shells by adsorbing cationic anionic chitosan derivatives a stepwise, layer-by-layer, procedure carried out an aqueous medium under mild conditions. films also deposited lipid bilayer the interactions studied using ellipsometry atomic force microscopy. covered with stored at 4 °C for 24 h express viability comparable to that control sample incubated 37 °C, while unprotected same conditions do not show viability. It is shown shell protects against damaging effect storage. Such nanocoatings provide protection, mechanical stability, support cell membrane, ensuring penetration small molecules such as nutrients/gases what essential