Inhibition of synaptically evoked cortical acetylcholine release by adenosine: an in vivo microdialysis study in the rat.

摘要: Abstract The release of cortical acetylcholine from the intracortical axonal terminals cholinergic basal forebrain neurons is closely associated with electroencephalographic activity. One factor which may act to reduce and promote sleep adenosine. Using in vivo microdialysis, we examined effect adenosine selective receptor agonists antagonists on evoked by electrical stimulation pedunculopontine tegmental nucleus urethane anesthetized rats. All drugs were administered locally within cortex reverse dialysis. None tested altered cortex. Adenosine significantly reduced efflux a concentration-dependent manner. This was mimicked A 1 agonist N 6 -cyclopentyladenosine blocked antagonist 8-cyclopentyl-1,3-dipropylxanthine (DPCPX). 2A 2-[ p -(2-carboxyethyl)-phenethylamino]-5′- -ethylcarboxamidoadenosine hydrochloride (CGS 21680) did not alter even presence DPCPX. Administered alone, neither DPCPX nor non-selective caffeine affected efflux. Simultaneous delivery uptake inhibitors dipyridamole S -(4-nitrobenzyl)-6-thioinosine release, this simultaneous administration caffeine. These data indicate that activation inhibits while does influence Such inhibition contribute an increased propensity during prolonged wakefulness.