Expression of Human Prohormone Convertase PC2 in a Baculovirus-Insect Cell System
作者： Margaret Fahnestock , Weijia Zhu
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摘要: PC2 is a member of the eukaryotic family subtilisin-related proprotein convertases which are thought to be involved in intracellular proteolytic processing prohormones and proneuropeptides. The presence only small amounts secretory granules certain mammalian neuroendocrine cell types has made characterization further study this enzyme difficult. We report here expression proteolytically active human protein insect cell-baculovirus system. Human expressed cells calcium-dependent at neutral pH. In cells, was found intracellularly as 75-kDa 71-kDa proteins. Both 73-kDa 68-kDa forms were conditioned medium, but no activity detected. demonstrated soluble inhibitor infected-cell medium may block activity.
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