Possible Mechanism for Formation and Regulation of the Palmitate-Induced Cyclosporin A-Insensitive Mitochondrial Pore

摘要: The mechanism of the palmitate-induced opening mitochondrial Ca2+-dependent cyclosporin A (CsA)-insensitive pore was studied, as well influence on this process well-known modulators CsA-sensitive pore. Palmitic acid, which can bind Ca2+ with high affinity, induced CsA-insensitive swelling mitochondria, whereas palmitoleic and 2-bromopalmitic acids, have no such affinity for Ca2+, failed to induce opening. palmitate mitochondria not affected by a inhibitor (ADP) an activator (inorganic phosphate, Pi). However, inhibited physiological concentrations ATP ([I]50 = 1.3 mM), but 100 µM increased 30% rate if had been added earlier. effects (inhibition activation) manifested themselves from different sides inner membrane. Mg2+ [I]50 0.8 mM. It is concluded that palmitic acid induces due its ability complexing Ca2+. possible formation some are discussed.