Preclinical Validation of Multilevel Intraparenchymal Stem Cell Therapy in the Porcine Spinal Cord.

摘要: BACKGROUND Although multiple clinical trials are currently testing different stem cell therapies as treatment alternatives for many neurodegenerative diseases and spinal cord injury, the optimal injection parameters have not yet been defined. OBJECTIVE To test cord's tolerance to increasing volumes numbers of injections in pig. METHODS Twenty-seven female Gottingen minipigs received human neural progenitor using a stereotactic platform device. Cell transplantation groups 1 5 (5-7 pigs each) was undertaken with intent assessing safety an volume escalation (10, 25, 50 µL) number (20, 30, 40 injections). Motor function general morbidity were assessed 21 days. Full necropsy performed; cords analyzed graft survival microscopic tissue damage. RESULTS No mortality or permanent surgical complications observed during 21-day study period. All animals returned preoperative baseline within 14 days, showing complete motor recovery. The histological analysis showed that there no significant decrease neuronal density between groups, engraftment ranged from 12% 31% depending on paradigm. However, damage identified when injecting large into (50 μL). CONCLUSION This series supports functional various gives critical insight important thresholds. These results relevant all translational programs delivering therapeutics cord.