Long-term study of Cryptosporidium prevalence on a lowland farm in the United Kingdom.

摘要: Abstract A longitudinal sample survey testing for Cryptosporidium in livestock and small wild mammals conducted over 6 years (1992–1997) on a lowland farm Warwickshire, England, has shown the parasite to be endemic persistently present all mammalian categories. Faecal samples were taken throughout year oocysts concentrated by formal ether sedimentation method detection immunofluorescence staining using commercially available genus specific monoclonal antibody. parvum was identified morphology measurement of modified Ziehl-Neelsen stained oocysts. C. muris rarely found andersoni never detected livestock. from 3721 individuals gave cumulative 6-year prevalences as follows: bull beef, 3.6%; dairy cows, 3.5%; ewes, 6.4%; horses, 8.9%; calves (home bred), 52%; (bought-in) 23.2%; lambs, 12.9%; (rodents) living around buildings, 32.8%; (mainly rodents) areas pasture, 29.9%. Animal categories with highest also shed average oocyst numbers per gram faeces (ranging 1.4×103 beef 1.1×105 calves). Analysis annual seasonal data each animal category revealed that patterns infection variable sporadic; this means short-term sampling likely provide true or representative picture. Seasonally combined adult livestock, young showed three tended have autumn. Calves separated their dams within 24 h birth yet high prevalence most despite low herd. It is possible coincidence autumn mice main period rearing contributed latter. The farming estate used teach students agriculture took pride good land management husbandry practices produced well fed healthy may represent, therefore, baseline, lowest levels expected, production United Kingdom.