Establishment of a pancreatic beta cell line that retains glucose-inducible insulin secretion: special reference to expression of glucose transporter isoforms
作者： JUN-ICHI MIYAZAKI , KIMI ARAKI , EIJI YAMATO , HIROSHI IKEGAMI , TOMOICHIRO ASANO
关键词:
摘要: Two cell lines have been established from insulinomas obtained by targeted expression of the simian virus 40 T antigen gene in transgenic mice. These lines, designated MIN6 and MIN7, produce insulin morphological characteristics pancreatic beta cells. cells exhibit glucose-inducible secretion comparable with cultured normal mouse islet cells, whereas MIN7 do not. Both liver-type glucose transporter (GT) mRNA at high level. Brain-type GT is also present considerable level but barely detectable suggesting that exclusive related to secretion. not express either major histocompatibility (MHC) class I or II antigens on surface. However, treatment interferon-gamma induces levels MHC antigens, a combination tumor necrosis factor-alpha results emphasize line retains physiological The will be especially useful analyze molecular mechanisms which regulate response extracellular concentrations. We discuss possible role isoforms sensing
oup.com
endojournals.org参考文章(36)
G A Praz, P A Halban, C B Wollheim, B Blondel, A J Strauss, A E Renold, Regulation of immunoreactive-insulin release from a rat cell line (RINm5F) Biochemical Journal. ,vol. 210, pp. 345- 352 ,(1983) , 10.1042/BJ2100345
M Welsh, N Scherberg, R Gilmore, D F Steiner, Translational control of insulin biosynthesis. Evidence for regulation of elongation, initiation and signal-recognition-particle-mediated translational arrest by glucose. Biochemical Journal. ,vol. 235, pp. 459- 467 ,(1986) , 10.1042/BJ2350459
H.S. Tager, A.H. Rubenstein, D.F. Steiner, [28] Methods for the assessment of peptide precursors. Studies on insulin biosynthesis Methods in Enzymology. ,vol. 37, pp. 326- 345 ,(1975) , 10.1016/S0076-6879(75)37030-4
K Ozato, D H Sachs, Monoclonal antibodies to mouse MHC antigens. III. Hybridoma antibodies reacting to antigens of the H-2b haplotype reveal genetic control of isotype expression. Journal of Immunology. ,vol. 126, pp. 317- 321 ,(1981)
K. Ozato, A. Rein, J.-I. Miyazaki, T. Silverman, G. Bratthauer, B. Orrison, J. Langloss, Establishment of cell lines from somite stage mouse embryos and expression of major histocompatibility class I genes in these cells. Journal of Immunology. ,vol. 140, pp. 4378- 4387 ,(1988)
R D Gerard, Y Gluzman, New host cell system for regulated simian virus 40 DNA replication. Molecular and Cellular Biology. ,vol. 5, pp. 3231- 3240 ,(1985) , 10.1128/MCB.5.11.3231
J Miller, R N Germain, Efficient cell surface expression of class II MHC molecules in the absence of associated invariant chain. Journal of Experimental Medicine. ,vol. 164, pp. 1478- 1489 ,(1986) , 10.1084/JEM.164.5.1478
R. F. Santerre, R. A. Cook, R. M. Crisel, J. D. Sharp, R. J. Schmidt, D. C. Williams, C. P. Wilson, Insulin synthesis in a clonal cell line of simian virus 40-transformed hamster pancreatic beta cells Proceedings of the National Academy of Sciences of the United States of America. ,vol. 78, pp. 4339- 4343 ,(1981) , 10.1073/PNAS.78.7.4339
IAIN L. CAMPBELL, KATJA BIZILJ, PETER G. COLMAN, BERNARD E. TUCH, LEONARD C. HARRISON, Interferon-γ Induces the Expression of HLA-A,B,C but Not HLA-DR on Human Pancreatic β-Cells* The Journal of Clinical Endocrinology and Metabolism. ,vol. 62, pp. 1101- 1109 ,(1986) , 10.1210/JCEM-62-6-1101
Bernard Thorens, Hemanta K. Sarkar, H.Ronald Kaback, Harvey F. Lodish, Cloning and functional expression in bacteria of a novel glucose transporter present in liver, intestine, kidney, and β-pancreatic islet cells Cell. ,vol. 55, pp. 281- 290 ,(1988) , 10.1016/0092-8674(88)90051-7