Application of TaqMan Low-Density Arrays for Simultaneous Detection of Multiple Respiratory Pathogens
作者： Maja Kodani , Genyan Yang , Laura M. Conklin , Tatiana C. Travis , Cynthia G. Whitney
DOI: 10.1128/JCM.02270-10
关键词:
摘要: The large and growing number of viral bacterial pathogens responsible for respiratory infections poses a challenge laboratories seeking to provide rapid comprehensive pathogen identification. We evaluated novel application the TaqMan low-density array (TLDA) cards real-time PCR detection 21 respiratory-pathogen targets. performance TLDA was compared that individual (IRTP) assays with same primers probes using (i) nucleic acids extracted from strains 66 closely related viruses bacteria (ii) 292 clinical specimens. With spiked samples, were about 10-fold less sensitive than IRTP assays. By specimens generate 2,238 paired assays, card exhibited 89% sensitivity (95% confidence interval [CI], 86 92%; range per target, 47 100%) 98% specificity CI, 97 99%; 85 overall as gold standard threshold cycle (CT) cutoff 43. approach offers promise simultaneous identification multiple outbreak investigations disease surveillance.
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sci-hub.se 参考文章(28)
G. Yang, R. Benson, T. Pelish, E. Brown, J.M. Winchell, B. Fields, Dual detection of Legionella pneumophila and Legionella species by real-time PCR targeting the 23S-5S rRNA gene spacer region. Clinical Microbiology and Infection. ,vol. 16, pp. 255- 261 ,(2010) , 10.1111/J.1469-0691.2009.02766.X
Adam Steg, Wenquan Wang, Carmelo Blanquicett, Jessica M Grunda, Isam A Eltoum, Kangsheng Wang, Donald J Buchsbaum, Selwyn M Vickers, Suzanne Russo, Robert B Diasio, Andra R Frost, Al F LoBuglio, William E Grizzle, Martin R Johnson, None, Multiple Gene Expression Analyses in Paraffin- Embedded Tissues by TaqMan Low-Density Array Application to Hedgehog and Wnt Pathway Analysis in Ovarian Endometrioid Adenocarcinoma The Journal of Molecular Diagnostics. ,vol. 8, pp. 76- 83 ,(2006) , 10.2353/JMOLDX.2006.040402
Genyan Yang, Dean E. Erdman, Maja Kodani, John Kools, Michael D. Bowen, Barry S. Fields, Comparison of commercial systems for extraction of nucleic acids from DNA/RNA respiratory pathogens. Journal of Virological Methods. ,vol. 171, pp. 195- 199 ,(2011) , 10.1016/J.JVIROMET.2010.10.024
Larry G. Reimer, Karen C. Carroll, Role of the Microbiology Laboratory in the Diagnosis of Lower Respiratory Tract Infections Clinical Infectious Diseases. ,vol. 26, pp. 742- 748 ,(1998) , 10.1086/514583
J. M. Winchell, K. A. Thurman, S. L. Mitchell, W. L. Thacker, B. S. Fields, Evaluation of Three Real-Time PCR Assays for Detection of Mycoplasma pneumoniae in an Outbreak Investigation Journal of Clinical Microbiology. ,vol. 46, pp. 3116- 3118 ,(2008) , 10.1128/JCM.00440-08
M KLEEMOLA, J NOKSOKOIVISTO, E HERVA, R SYRJANEN, M LAHDENKARI, T KILPI, T HOVI, Is there any specific association between respiratory viruses and bacteria in acute otitis media of young children Journal of Infection. ,vol. 52, pp. 181- 187 ,(2006) , 10.1016/J.JINF.2005.05.012
Peter Jacoby, Kelly Watson, Jacinta Bowman, Amanda Taylor, Thomas V Riley, David W Smith, Deborah Lehmann, Kalgoorlie Otitis Media Research Project Team, Modelling the co-occurrence of Streptococcus pneumoniae with other bacterial and viral pathogens in the upper respiratory tract. Vaccine. ,vol. 25, pp. 2458- 2464 ,(2007) , 10.1016/J.VACCINE.2006.09.020
Stephanie L. Mitchell, Sona Budhiraja, Kathleen A. Thurman, W. Lanier Thacker, Jonas M. Winchell, Evaluation of two real-time PCR chemistries for the detection of Chlamydophila pneumoniae in clinical specimens Molecular and Cellular Probes. ,vol. 23, pp. 309- 311 ,(2009) , 10.1016/J.MCP.2009.07.005
Kathleen M. Tatti, Kai-Hui Wu, Maria Lucia Tondella, Pamela K. Cassiday, Margaret M. Cortese, Patricia P. Wilkins, Gary N. Sanden, Development and evaluation of dual-target real-time polymerase chain reaction assays to detect Bordetella spp. Diagnostic Microbiology and Infectious Disease. ,vol. 61, pp. 264- 272 ,(2008) , 10.1016/J.DIAGMICROBIO.2008.02.017
Angela B Y Hui, Wei Shi, Paul C Boutros, Naomi Miller, Melania Pintilie, Tony Fyles, David McCready, Derek Wong, Kate Gerster, Igor Jurisica, Linda Z Penn, Fei-Fei Liu, Robust global micro-RNA profiling with formalin-fixed paraffin-embedded breast cancer tissues Laboratory Investigation. ,vol. 89, pp. 597- 606 ,(2009) , 10.1038/LABINVEST.2009.12