Mapping of the human lambda immunoglobulin variable gene subgroup 1

摘要: We describe the cloning and mapping of 20 putative members IGLV subgroup 1. These gene segments are contained on 26 phage clones which fall into 7 contigs plus one solitary phage. This represents approximately 240 kilobases (kb) cloned DNA. Like IGLC segments, were found to be oriented proximal distal chromosome, indicating IGL somatic rearrangement is by deletion. The placed a long-range map locus, covers at least 800 kb. Clones further ordered pulsed field gel electrophoresis analysis B-cell lines known produce IGL-containing immunoglobulins. DNA deletions ranged from 120 570