Incorporation of fucose and glucosamine into cell bound and medium released macromolecules
作者： Heinz Herrmann , Amalia S. Havaranis , Thomas C. Doetschman
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摘要: (1) Determinations were carried out on the incorporation of fucose-6-[3H] and glucosamine-6- [3H] into trichloracetic acid insoluble macromolecules which remained bound to cells or released medium chick embryo muscle cell cultures. The radioactivity determined in was corrected for unspecific binding label components medium. (2) During an period six hours per microgram DNA with fucose as is about twice high With glucosamine much incorporated than macromolecules. (3) increases during a culture three days but increase ceases at different times this when material. (4) An density slighter extent that glucosamine. Reduction by addition cytosine arabinoside does not decrease DNA. (5) effect changes fibroblast/myoblast ratios examined. No significant observed ratio 10–30% fibroblasts control cultures after sedimentation maintained complete Marked without protein components. Under these conditions well
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