Application of Enzymatic Extracts from a CALB Standard Strain as Biocatalyst within the Context of Conventional Biodiesel Production Optimization.
作者： Carlos Luna , Diego Luna , Felipa M Bautista , Rafael Estevez , Juan Calero
DOI: 10.3390/MOLECULES22112025
关键词:
摘要: The application of biocatalysts in the transesterification process triglycerides (TG) allows integrating glycerol form monoglyceride (MG), sharply increasing yield and environmental sustainability conventional biodiesel production process. This is known as Ecodiesel. To overcome inconvenient high cost currently employed highly purified commercial enzymes, use scarcely extracts obtained from standard strains same species lipases applied this being investigated. Thus, Candidaantarctica type B (CALB) was chosen to determine optimal conditions culture yeast. strain Spanish Type Microbial Cultures Collection (CECT) has been used carry out several studies elucidate its optimum growth conditions. Through a lyophilization with prior dialysis liquid cultures, enzymatic were obtained. different cultures have 1,3-selective reaction sunflower oil ethanol obtain Ecodiesel (FAEE + MG). Selectivity yields by gas chromatography. Acceptable are during time well successive reactions, demonstrating feasibility using these CALB biocatalysts.
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