The Epstein–Barr virus oncogene product, latent membrane protein 1, induces the downregulation of E-cadherin gene expression via activation of DNA methyltransferases

摘要: The latent membrane protein (LMP1) of Epstein–Barr virus (EBV) is expressed in EBV-associated nasopharyngeal carcinoma, which notoriously metastatic. Although it established that LMP1 represses E-cadherin expression and enhances the invasive ability carcinoma cells, mechanism underlying this repression remains to be elucidated. In study, we demonstrate induces activity DNA methyltransferases 1, 3a, 3b, using real-time reverse transcription–PCR enzyme assay. This results hypermethylation promoter down-regulation gene expression, as revealed by methylation-specific PCR, Western blotting data. methyltransferase inhibitor, 5′-Aza-2′dC, restores LMP1-expressing turn blocks cell migration ability, demonstrated Transwell Our findings suggest down-regulates cellular methylation machinery.