High Frequency of Promoter Hypermethylation of RASSF1A in Nasopharyngeal Carcinoma
作者： Kwok-Wai Lo , Joseph Kwong , Angela Bik-Yu Hui , Sylvia Yat-Yee Chan , Ka-Fai To
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摘要: We have investigated the genetic and epigenetic changes of a newly isolated tumor suppressor gene on 3p21.3, RASSF1A, in nasopharyngeal carcinoma (NPC). Four xenografts, four cell lines 21 primary tumors were examined. Promoter hypermethylation 5'CpG island RASSF1A was detected 4 (100%) 3 (75%) lines, 14 (66.7%) but not normal epithelia. Mutations found 2 (9.5%) tumors. In xenografts with extensive methylation, no expression found. After treatment 5'-aza-2'deoxycytidine, reexpression demethylation NPC line. These findings suggest that promoter may participate transcriptional inactivation NPC. The high incidence alterations it is critical target chromosome 3p21.3 involved development
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