Helix dynamics in a membrane transport protein: comparative simulations of the glycerol-3-phosphate transporter and its constituent helices.
作者： Robert S. G. D'rozario , Mark S. P. Sansom
DOI: 10.1080/09687680802549113
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摘要: The glycerol-3-phosphate transporter (GlpT) is a member of the major facilitator superfamily (MFS). GlpT an organic phosphate/inorganic phosphate antiporter. It shares similar fold with other MFS transporters (e.g. LacY and EmrD) consisting 12 transmembrane (TM) helices which form two domains (each six TM helices) surrounding central ligand-binding cavity. (especially cavity-lining contain large number proline glycine residues, may aid in conformational changes believed to underline transport mechanism. Molecular dynamics simulations phospholipid bilayer have been used compare properties isolated those intact protein. Analysis these focuses on role proline-induced flexibility helices. Our results are consistent proposed rocker switch mechanism for by GlpT. In particular, highlight (H4, H5, ...
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