MEK and TGF-beta Inhibition Promotes Reprogramming without the Use of Transcription Factor

摘要: The possibility of replacing the originally discovered and widely used DNA reprogramming transcription factors is stimulating enormous effort to identify more effective compounds that would not alter genetic information. Here, we describe generation induced pluripotent stem cells (iPSc) from head-derived primary culture mouse embryonic using small chemical inhibitors MEK TGF-beta pathways without delivery exogenous factors. These iPSc express standard pluripotency markers retain their potential differentiate into all germ layers. Our data indicate neural might have while neither same cultivated over five passages in vitro nor a cell population derived adult brain possesses this capacity. results reveal for molecules functionally replace routinely lift veil on molecular regulation controlling pluripotency. conditions described here could provide platform upon which other genome non integrative safer processes be developed. This work also shows novel developing cells.