Sensing Single Protein Molecules with Solid-State Nanopores

摘要: This chapter is focused on the development of experiments and theory using solid-state nanopores for sensing single protein molecules in their native unfolded states. Proteins serve diverse roles such as transport carriers, catalysts, molecular motors, cellular structural support, others that make life possible. Because these widely differing roles, proteins have an enormously set shapes, sizes, charge structures compared to polynucleic acids. Solid-state are particularly suitable characterizing because they can be fabricated with adjustable dimensions stable under conditions denature proteins. describes nanopore experimental setup, signal recording, data analysis, basic principles related connecting electrical properties Examples results illustrate ability differentiate folded Native-state translocation follows biased one-dimensional diffusion charged particles sensitive size charge. Due heterogeneous sequence polypeptides, obey a coupled electrophoretic thermally activated process specific. The concludes discussion future directions open challenges characterization nanopores.