Molecular characterization and differential mRNA expression profiling of Toll-like receptor-2 gene in Vechur (Bos indicus) and crossbred (Bos indicus X Bos taurus) cattle of Kerala in response to anthrax vaccination

作者: P.N. Shivakumara , T.V. Aravindakshan , Thomas Naicy , K. Anilkumar , R. Uma

DOI: 10.1016/J.MGENE.2018.01.003

关键词:

摘要: Abstract Toll like receptors (TLRs) are type 1 transmembrane proteins expressed in almost all cell types and activate the innate immune system. The present study involved characterization differential transcriptional expression profiling of Toll-like receptor-2 gene Vechur (Bos indicus) crossbred indicus X Bos taurus) cattle Kerala response to anthrax vaccination. Sequence analysis revealed 2355 bp long TLR2 open reading frame encoding 784 amino acids with a signal peptide 20 acid residues. sequence 96–99% identity other ruminant species. At level, 94–99% similarity was observed livestock Seven nucleotide variations were when compared Indian 17 taurus. five non-synonymous mutations as (GenBank Acc. No. EU413951.1 ) p.Gln149Pro, p.Ile335Thr, p.Lys.337Arg, p.Ser345Asn p.Met605Thr. eight non synonymous taurus NM174197.2) p.Asp63Glu, p.Gly68Ser, p.Ile111Val, p.Phe127Leu, p.His326Gln, p.Asn417Ser, p.Arg563His p.His665Gln. This is first report about full-length cDNA single cattle, smallest world. prolonged upregulation mRNA Crossbred post vaccination suggestive greater this native exotic cattle.

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