Evaluation and comparison of the constitutive expression levels of Toll-like receptors 2, 3 and 7 in the peripheral blood mononuclear cells of Tharparkar and crossbred cattle
作者： Lakshya Veer Singh , Shikha Saxena , Smita Gupta , Shishir Kumar Gupta , G. Ravi Kumar
DOI: 10.14202/VETWORLD.2014.209-212
关键词: Expression pattern 、 TLR2 、 Gene expression 、 Biology 、 Receptor 、 Crossbred cattle 、 Veterinary medicine 、 Tharparkar cattle 、 Crossbreed 、 Peripheral blood mononuclear cell
摘要: Aim: This study was undertaken to assess the differential expression levels of toll-like receptors (TLRs) 2, 3 and 7 in peripheral blood mononuclear cells (PBMCs) isolated from Tharparkar Crossbred cattle belonging different regions India. Materials Methods: PBMCs were samples Indian Veterinary Research Institute (IVRI) farm (n=30); Suratgarh (n=61); Jaipur (n=8) cross breed (n=47). RNA cDNA synthesized using random hexamers. The profiles TLR estimated by real-time PCR normalized ‚-actin. Results: Suratgarh, exhibited a significantly higher (p<0.05) constitutive TLR2, TLR3 TLR7 genes as compared IVRI or well crossbred Jaipur. showed all TLRs than IVRI. Conclusion: Our indicates, are for However, basal three Results also indicate that show regional variation pattern TLRs.
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