An siRNA against JC virus (JCV) agnoprotein inhibits JCV infection in JCV-producing cells inoculated in nude mice

摘要: JC virus (JCV) is the etiological agent of demyelinating disease progressive multifocal leukoencephalopathy (PML). Because JCV has a very narrow host range, it been difficult to develop an animal model infection; as result, no effective therapy for PML established. In this study, we have tried create that replaces in vivo infection. As obtained stable persistence JCV-infected human cells mouse brain by inoculating virus-infected into nude mice brains. model, were well preserved brains 2 weeks. We then treated JCV-injected with siRNA against agnoprotein known be inhibitor infection vitro. A highly purified type I collagen, atelocollagen, was used carrier siRNA. The inhibited expression protein inoculated brain, compared medium containing only atelocollagen placebo. Thus, combination and might candidate therapeutic treatment