Effect of a biofilm‐degrading enzyme from an oral pathogen in transgenic tobacco on the pathogenicity of Pectobacterium carotovorum subsp. carotovorum

摘要: The hypothesis that dispersin B (DspB), an enzyme from the periodontal pathogen Aggregatibacter actinomycetemcomitans degrades extracellular matrix polysaccharide PGA, will inhibit biofilm formation of soft rot Pectobacterium carotovorum subsp. in infected plants was tested by constitutive expression DspB tobacco plants. All transgenic expressed properly folded and active enzyme, although at different levels. In virulence assays, even plant line D10, which produced a low level compared to other lines, showed significant resistance against P. carotovorum carotovorum, suggesting could be valuable agent for biological control infection crop