Intracellular Nanoparticle Aggregation as a Mechanism for Inducing Apoptosis in Breast Cancer Cells
作者： Szu-Wen Wang
DOI: 10.21236/ADA542536
关键词:
摘要: Abstract : The current paradigm of drug delivery using nanotechnology has been focused on coupling chemotherapeutic molecules to nanoparticulate systems. In this work, we propose that pH-triggered aggregation alone nanoparticles (that are non-toxic when not aggregated) can induce death in breast cancer cells. We hypothesize non-toxic, protein nanoparticles, internalized by endocytosis and triggered aggregate inside cells, will be cytotoxic them. examine (a) the extent which pH-induced intracellular is cytotoxic, (b) whether a pH-responsive nanoparticle yields synergistic effects toxicity. Although was Concept Award only funded one year proposed research, one-year, no-cost extension granted complete remaining tasks. This report reflects one-year update report.
sci-hub.st 参考文章(23)
Ulrich Beyer, Barbara Rothen‐Rutishauser, Clemens Unger, Heidi Wunderli‐Allenspach, Felix Kratz, Differences in the intracellular distribution of acid-sensitive doxorubicin-protein conjugates in comparison to free and liposomal formulated doxorubicin as shown by confocal microscopy. Pharmaceutical Research. ,vol. 18, pp. 29- 38 ,(2001) , 10.1023/A:1011018525121
B. Devika Chithrani, Arezou A. Ghazani, Warren C. W. Chan, Determining the size and shape dependence of gold nanoparticle uptake into mammalian cells. Nano Letters. ,vol. 6, pp. 662- 668 ,(2006) , 10.1021/NL052396O
Mohsen Shadidi, Mouldy Sioud, Selective targeting of cancer cells using synthetic peptides. Drug Resistance Updates. ,vol. 6, pp. 363- 371 ,(2003) , 10.1016/J.DRUP.2003.11.002
Hyuk Sang Yoo, Tae Gwan Park, Biodegradable polymeric micelles composed of doxorubicin conjugated PLGA–PEG block copolymer Journal of Controlled Release. ,vol. 70, pp. 63- 70 ,(2001) , 10.1016/S0168-3659(00)00340-0
Elizabeth R. Gillies, Jean M. J. Fréchet, pH-Responsive copolymer assemblies for controlled release of doxorubicin. Bioconjugate Chemistry. ,vol. 16, pp. 361- 368 ,(2005) , 10.1021/BC049851C
Matthew R Dreher, Drazen Raucher, Narayanan Balu, O Michael Colvin, Susan M Ludeman, Ashutosh Chilkoti, Evaluation of an elastin-like polypeptide-doxorubicin conjugate for cancer therapy Journal of Controlled Release. ,vol. 91, pp. 31- 43 ,(2003) , 10.1016/S0168-3659(03)00216-5
Mahesh D. Chavanpatil, Ayman Khdair, Brigitte Gerard, Corbin Bachmeier, Donald W. Miller, Malathy P. V. Shekhar, Jayanth Panyam, Surfactant-polymer nanoparticles overcome P-glycoprotein-mediated drug efflux. Molecular Pharmaceutics. ,vol. 4, pp. 730- 738 ,(2007) , 10.1021/MP070024D
C S Pichot, S M Hartig, L Xia, C Arvanitis, D Monisvais, F Y Lee, J A Frost, S J Corey, Dasatinib synergizes with doxorubicin to block growth, migration, and invasion of breast cancer cells. British Journal of Cancer. ,vol. 101, pp. 38- 47 ,(2009) , 10.1038/SJ.BJC.6605101
Gea Speelmans, Rutger W. H. M. Staffhorst, Ben de Kruijff, Frits A. de Wolf, Transport studies of doxorubicin in model membranes indicate a difference in passive diffusion across and binding at the outer and inner leaflets of the plasma membrane. Biochemistry. ,vol. 33, pp. 13761- 13768 ,(1994) , 10.1021/BI00250A029
Kamal K Upadhyay, Anant N Bhatt, Anil K Mishra, Bilikere S Dwarakanath, Sanyog Jain, Christophe Schatz, Jean-François Le Meins, Abdullah Farooque, Godugu Chandraiah, Amit K Jain, Ambikanandan Misra, Sebastien Lecommandoux, None, The intracellular drug delivery and anti tumor activity of doxorubicin loaded poly(γ-benzyl L-glutamate)-b-hyaluronan polymersomes Biomaterials. ,vol. 31, pp. 2882- 2892 ,(2010) , 10.1016/J.BIOMATERIALS.2009.12.043