Enzymatic synthesis of S-adenosyl-l-methionine on the preparative scale

摘要: Abstract The problems inherent in the enzymatic and chemical synthesis of S- adenosyl- l -methionine (SAM) led us to develop an efficient, simple method for large amounts labeled SAM. Previously, we reported that problem product inhibition E. coli SAM synthetase encoded by metK gene was successfully overcome presence sodium p-toluenesulfonate (pTsONa). This research has now been expanded demonstrate this enzyme can also be adding a high concentration β-mercaptoethanol (βME), acetonitrile, or urea. In addition, recombinant strain constructed expresses yeast sam2 gene. does not have seen with enzyme. Complete conversion 10 mM methionine achieved incubations either 1 molar potassium ion additives such as βME, urea, pTsONa. used generate situ use multi-enzymatic precorrin 2.