A synergistic effect on the production of S-adenosyl-l-methionine in Pichia pastoris by knocking in of S-adenosyl-l-methionine synthase and knocking out of cystathionine-β synthase

摘要: Two strategies of metabolic engineering have been used, individually or combined, to alter the flux improve production S-adenosyl-L-methionine (SAM) in Pichia pastoris. One is over expressing SAM synthase by knock-in technique, other disruption cystathionine-beta (CBS) knock-out technique. Strain Gsam with ectopic gene produced 20 times comparing starter strain GS115. Disruption CBS GS115 only doubled its production. However, results a robust increase production, more than 56 Thus, we report for first time synergistic effect on yeast combination and techniques. Furthermore, optimize cultural conditions genetically modified Gsam-cbs produce SAM. The maximum yield reaches 3.6g/L shake flask 13.5g/L 5L fermentor, indicating that it could be used industrial fermentation large scale