Enhanced S-adenosyl-l-methionine production in Saccharomyces cerevisiae by spaceflight culture, overexpressing methionine adenosyltransferase and optimizing cultivation

摘要: Aims: S-adenosyl-l-methionine (SAM) is an important biochemical molecule with great potential in the pharmacological and chemotherapeutic fields. In this study, our aims were to enhance SAM production Saccharomyces cerevisiae. Methods Results:  Through spaceflight culture, a SAM-accumulating strain, S. cerevisiae H5M147, was isolated found produce 86·89% more than its ground control strain H5. Amplified fragment length polymorphism (AFLP) analysis demonstrated that there genetic variations between H5M147 control. recombinant DNA technology, heterologous gene encoding methionine adenosyltransferase integrated into genome of H5M147. The H5MR83 selected because increased by 42·98% when compared Furthermore, cultivation conditions optimized using one-factor-at-a-time Taguchi methods. Under optimal conditions, yielded 7·76 g l−1 shake flask, increase 536·07% 9·64 g l−1 produced fermenter cultivation. Conclusions:  A new H5MR83, obtained through culture modification. relative high level study. Significance Impact Study:  comprehensive application multiple methods including modification optimizing cultivation, yield could be 6·4 times flask cultures, significant improvement on previously reported results. period 84 h, which shorter cerevisiae has considerable for use industrial applications.