Sensitivity of Five Rapid HIV Tests on Oral Fluid or Finger-Stick Whole Blood: A Real-Time Comparison in a Healthcare Setting

摘要: Background: Health authorities in several countries recently recommended the expansion of human immunodeficiency virus (HIV) antibody testing, including use rapid tests. Several HIV tests are now licensed Europe but their sensitivity on total blood and/or oral fluid routine healthcare settings is not known. Methods and Findings: 200 adults with documented HIV-1 (n=194) or HIV-2 infection (n=6) were prospectively screened five using either (OF) finger-stick whole (FSB). The OraQuick Advance HIV1/2H was first applied to OF then FSB, while other following order: Vikia 1/2H, Determine 1–2H, DetermineH HIV-1/2 Ag/Ab ComboH INSTI HIV-1/HIV-2H. Tests negative FSB repeated paired serum samples. Twenty randomly selected HIV-seronegative subjects served as controls, results read blindly. Most patients had subtype B (63.3%) most antiretroviral therapy (68.5%). Sensitivity 86.5%, 94.5%, 98.5%, 94.9%, 95.8% 99% respectively, OF, Vikia, Determine, Ag/ Ab Combo (p,0.0001). less sensitive than (p=0.008). Among six three more tests, two recent four undetectable plasma viral load. When positive all compared who at least one test, only a RNA level ,200 cp/ml significantly associated false-negative result (p=0.009). 33 serum, (5 OraQuick, 1 INSTI) positive. better (97.5%, p=0.04; 100%, p=0.004; p=0.02, respectively). Conclusion: evaluated setting, serum.