Hepatitis C virus and liver disease: Global transcriptional profiling and identification of potential markers

摘要: Abstract Microarray analysis of RNA from hepatitis C virus (HCV)-infected cirrhotic livers was performed to identify a gene expression signature liver disease. The levels approximately 13,600 genes were analyzed using surgical material and core biopsy specimens HCV-infected explants in comparison with reference samples normal nondiseased liver. In addition, compared each other determine physiologic variation expression. A set genes, including some associated stress, acute-phase immune response, hepatic stellate cell activation, had variable livers. These subtracted the sets differentially expressed To exclude cancer-related our marker sets, we that also hepatocellular carcinomas. resultant HCV- disease-associated provided molecular portrait several processes occurring It included (1) activated lymphocytes infiltrating liver, macrophages; (2) involved remodeling extracellular matrix-cell cell-cell interactions cytoskeleton rearrangements; (3) related anti-apoptotic pathway Bcl-2 signaling; (4) interferon response virus-host interactions. conclusion, microarray identified potential markers HCV-associated disease contributed rapidly expanding database experiments describing HCV pathogenesis. (H epatology 2003;38:1458-1467.)