The correct analysis of low-speed sedimentation equilibrium distributions recorded by the Rayleigh interference optical system in a Beckman XL-I ultracentrifuge

摘要: The molecular mass of ovalbumin, a well-characterized protein, has been determined from low-speed sedimentation equilibrium distributions recorded by the absorption and Rayleigh optical systems Beckman XL-I ultracentrifuge in order to assess reliability various procedures for analyzing interfero-metric records. Despite assertions contrary, present results demonstrate importance establishing concentration distribution terms absolute fringe displacement, J(r) versus r, before quantitative analysis is atttempted basic expression single solute: this consideration particularly important experiments where con-centration at meniscus sizeable relation difference across distribution. In that regard incorporation synthetic boundary experiment into protocol seems provide most reliable means ascertaining interferometric records experiments, an essential prerequisite characterization interacting systems.