MOLECULAR CHARACTERIZATION OF THE LINUSITIN-LIKE GENE FAMILY FROM FLAX

作者: Sabina Anžlovar , Kristina Gruden , Boris Rogelj , Borut Štrukelj , Marina Dermastia

DOI: 10.1086/499612

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摘要: Osmotin‐like proteins from the fifth class of pathogenesis‐related (PR‐5), including linusitin that we isolated flax seeds, have been characterized in many plant species. A cDNA library has constructed (Linum usitatissimum L.) seedlings to analyze osmotin‐like gene family flax. LIN1 was and found exhibit significant similarities genes encoding thaumatin Thaumatococcus daniellii. The deduced protein contains all 16 cysteine residues are conserved PR‐5 as well other important for antifungal activity. encodes a preprotein, which is subsequently processed into mature by removal an N‐terminal signal peptide. Southern blot analysis showed encoded few copies genome. intronless developmentally regulated, transcripts accumulate organ‐specific manner healthy...

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