A Critical Role for Sialylation in Cryoglobulin Activity of Murine IgG3 Monoclonal Antibodies

摘要: Cryoprecipitating IgG3 autoantibodies have been shown to play a significant role in the development of murine lupus-like autoimmune syndrome. However, structural basis cryoprecipitation still remains be defined. In view implication positively charged amino acid residues present variable regions cryoglobulin activity, we explored terminal sialic acids oligosaccharide side chains for cryogenic activity mAb. Comparative analysis different and non-cryogenic mAb showed an inverse correlation between extent sialylation activity. The inhibitory was further confirmed by demonstration enrichment less more sialylated cryoprecipitated noncryoprecipitated fractions, respectively, separated from four Significantly, contents latter fraction became comparable those Finally, observed that highly potent inhibition Our results thus suggest content negatively is one critical factors determine along with sequences regions.