Rapid and accurate determination of the potency of varicella vaccine by quantitative polymerase chain reaction
作者： Marsha S. Russell , Changgui Li , Louise Larocque , Junzhi Wang , Aaron Farnsworth
DOI: 10.1016/J.VACCINE.2011.09.017
关键词:
摘要: Abstract The potency of varicella vaccines is currently determined by a plaque assay technique, which usually takes seven days and laborious has considerable inter- intra-assay variability. Here, we report new for vaccine based on quantitative polymerase chain reaction in conjunction with much more efficient virus infection step. Potency results can be obtained within 24 h demonstrates acceptable accuracy reproducibility when compared the assay, relies manual counting plaques formed one week after viral infection. Using multiple lots from 7 manufacturers, found no significant difference infectivity between assay. optimized conditions are value determination due to its rapidity, high throughput capacity
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