Chromatin Decondensation and Nuclear Softening Accompany Nanog Downregulation in Embryonic Stem Cells

摘要: The interplay between epigenetic modification and chromatin compaction is implicated in the regulation of gene expression, it comprises one most fascinating frontiers cell biology. Although a complete picture still lacking, generally accepted that differentiation embryonic stem (ES) cells accompanied by selective condensation into heterochromatin with concomitant silencing, leaving access only to lineage-specific genes euchromatin. ES have been reported less condensed chromatin, as they are capable differentiating any type. However, pluripotency itself—even prior differentiation—is split state comprising naive which prime for differentiation. Here, we show decondense their course downregulating marker Nanog before initiate lineage commitment. We used fluorescence recovery after photobleaching, histone analysis paired novel, our knowledge, optical stretching method, significantly stiffer nucleus coupled globally more state. link this biophysical phenotype coinciding differences, including methylation, strong correlation nuclear stiffness expression Nanog. Besides having implications transcriptional sorting suggesting putative mechanosensing mechanism, physical differences point system-level regulatory role maintaining development.