A New Binding Motif for the Transcriptional Repressor REST Uncovers Large Gene Networks Devoted to Neuronal Functions

摘要: The repressor element 1 (RE1) silencing transcription factor (REST) helps preserve the identity of nervous tissue by neuronal genes in non-neural tissues. Moreover, an epithelial model tumorigenesis, loss REST function is associated with adhesion, suggesting aberrant expression REST-controlled encoding this property. To date, no adhesion molecules under control have been identified. Here, we used serial analysis chromatin occupancy to perform genome-wide identification REST-occupied target sequences (RE1 sites) a kidney cell line. We discovered novel REST-binding motifs and found that number RE1 sites far exceeded previous estimates. A large family targets proteins was identified, as were signature neuroendocrine tumors. Unexpectedly, considered exclusively non-neuronal also contained motif expressed neurons. This supports binding critical determinant phenotype.