Temporal response dynamics of Drosophila olfactory sensory neurons depends on receptor type and response polarity

摘要: Insect olfactory sensory neurons (OSN) express a diverse array of receptors from different protein families i.e. ionotropic (IR), gustatory (GR) and odorant (OR). It is well known that insects are exposed to plethora odor molecules vary widely in both space time under turbulent natural conditions. In addition divergent ligand specificities, these might also provide an increased range temporal dynamics sensitivities for the system. To test this, we challenged Drosophila OSNs with varying stimulus durations (10-2000 ms), repeated pulses key ligands at various frequencies (1-10 Hz). Our results show OR-expressing responded faster higher sensitivity short stimulations as compared IR- Gr21a-expressing OSNs. addition, could respond excitatory up 5 Hz, while IR-expressing required ~5x longer and/or concentrations similar frequencies. Nevertheless, did not exhibit adaptation stimulations, unlike OR Gr21a-OSNs. Both were unable resolve inhibitory fast ligands. These differences independent peri-receptor environment which expressed suggest receptor by given OSN affects its response transient, intermittent chemical stimuli. better resolving low dose, stimuli, more accurately long-lasting pulses. This diversity increases capacity insect system spatiotemporal signals environment.