Early effects of denervation on Ca(2+)-handling proteins in skeletal muscle.

摘要: The adaptive response of skeletal muscle fibres depends on a variety biological factors including loading conditions and neuromuscular activity. An extreme type atrophy-inducing change in contractile activity is represented by the physical disconnection between motor nerve its respective fibre unit. Since alterations have striking effect Ca(2+)-regulatory apparatus, we investigated fate key Ca(2+)-pump essential Ca(2+)-binding proteins extensor digitorum longus specimens two weeks after crush or complete denervation. In contrast to increased levels sarcalumenin, immunoblotting revealed that expression fast SERCA1 Ca(2+)-ATPase isoform drastically decreased calsequestrin slightly reduced. Analysis myosin heavy chain isoforms agreed with this result showed fast-to-slow shifting process following Hence, changes appear profound abundance pattern Ca(2+)-handling elements.