Measurement of the Oxidative DNA Lesion 8-Oxoguanine (8-oxoG) by ELISA or by High-Performance Liquid Chromatography (HPLC) with Electrochemical Detection.
作者： Danielle M. Drake , Aaron M. Shapiro , Peter G. Wells
DOI: 10.1007/978-1-4939-9182-2_21
关键词:
摘要: Reactive oxygen species (ROS) can oxidize cellular macromolecules like DNA, causing DNA damage. The most common form of damage is the 8-oxoguanine (8-oxoG) lesion, typically repaired by base excision repair (BER) pathway, which initiated enzyme oxoguanine glycosylase 1 (OGG1). ROS are produced endogenously and be enhanced environmental factors, such as xenobiotics, radiation, microbial pathogens. As a commonly used biomarker oxidative damage, 8-oxoG measured in two different ways described herein. Commercially available ELISA kits allow for easy detection while more difficult HPLC assays with UV electrochemical definitive identification quantification 8-oxoG.
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