Identification and characterization of transcriptional regulatory elements of the human aromatase cytochrome P450 Gene (CYP19)

摘要: Abstract Aromatase cytochrome P450, a member of the P450 gene super family, catalyzes conversion androgens to estrogens in form an enzyme-complex with NADPH-cytochrome reductase. Transcription aromatase (CYP19) is regulated part by tissue-specific promoters coupled alternative splicing mechanisms. The transcription human placenta governed promoter activity 5′ flanking region exon I.1, which mapped more than 40 kb upstream from translational start codon observed II. Transient expression analyses chimeric constructs containing sequences linked bacterial chloramphenicol acetyltransferase (CAT) BeWo choriocarcinoma cells localized cell-type specific enhancer element between −242 and −166 relative major cap site. DNase I footprinting transient indicate that it consists two sub-elements both are necessary for maximum enhancement transcription. In addition element, cis-acting important transcriptional response 12-O-tetradecanoylphorbol 13-acetate −2141 −2115. A nuclear factor binding identified as NF-IL6 (also termed LAP C/EBP β). using CAT sites establish involvement regulation CYP19.