Mechanism of activation of an N-ras gene in the human fibrosarcoma cell line HT1080.

摘要: A full length N-ras gene has been cloned from both the human fibrosarcoma cell line HT1080 and normal DNA. isolated will efficiently induce morphological transformation of NIH/3T3 cells in a transfection assay, whereas DNA no effect on cells. The coding regions have sequenced predicted amino acid sequence product is very similar to that c-Ha-ras1 c-Ki-ras2 products. By making chimeric molecules between two genes activating alteration localised single base change results an at position 61 p21 product.