Anti‐metastatic vaccination of tumor‐bearing mice with il‐2‐gene‐inserted tumor cells

摘要: IL-2 gene was introduced through retroviral vectors into the highly malignant and poorly immunogenic 3LL-D122 clone. Both high low D122-11-2 secretors showed elimination of tumorigenicity in syngeneic immune-competent mice; however, nude mice only secretor reduced as compared with parental D 122 cells. Also, following intravenous inoculation, generation metastases, whereas were metastatic These results seem to indicate that levels secreted by tumor cells are sufficient activate T cells, while higher needed order non-T-cell effectors. D122-IL-2 induced anti-tumor CTL, their sensitivity lytic activity these CTL similar D122 thus indicating elevation immunogenicity essentially due IL-2. In accordance induction, pre-immunization protected against subsequent challenge Moreover, immunization an “immunotherapy protocol” i.e., vaccination carrying established inactivated infectants, almost completely eliminated lung metastases providing a rationale for use transferred modality treatment metastasis.