Effect of ozonation on Spirulina platensis filaments by dynamic imaging particle analysis

摘要: Abstract Increasing interest in microalgal research calls for simple and automated tools to identify algal species' taxonomic level, examine their quality, monitor growth, determine size shape, enumerate them. We demonstrate the applicability of micro-flow imaging as an innovative tool count cells shape size, toward a future systematic species database, biofuel applications. Ozonation was used introduce changes cell morphology such oxidation, lysis agglomeration, which were characterized by imaging. Two ozone system configurations used—batch semi-batch—to oxidize four different Spirulina platensis samples, each presenting initial correlated culture growth stage, environmental conditions hydration–dehydration cells. The dose acted differently system: with batch (ozone 5–15 mg L−1), dominant action breakage spirulina filaments into smaller ones; semi-batch up ∼510 mg L−1), perforation walls increase filament transparency (measured mean intensity). study also showed influence stage on ozonated analysis.