Lentiviral gene transfer to the nonhuman primate brain.

摘要: Lentiviral vectors infect quiescent cells and allow for the delivery of genes to discrete brain regions. The present study assessed whether stable lentiviral gene transduction can be achieved in monkey nigrostriatal system. Three young adult Rhesus monkeys received injections a vector encoding marker β galatosidase (βGal). On one side brain, each multiple lentivirus into caudate putamen. opposite side, animal single injection aimed at substantia nigra. first two were sacrificed 1 month postinjection, while third was 3 months postinjection. Robust incorporation βGal seen striatum all three monkeys. Stereological counts revealed that 930,218; 1,192,359; 1,501,217 positive (n = 2) 1) later, respectively. Only had an placed directly nigra 187,308 βGal-positive identified this animal. induced only minor perivascular cuffing there no apparent inflammatory response resulting from injections. Double label experiments between 80 87% neurons. These data indicate robust striatal nigral occur nonhuman primate up months. Studies are now ongoing testing ability dopaminergic trophic factors augment system models Parkinson's disease.