Evaluation of a simple protein free medium that supports high levels of monoclonal antibody production
作者： Y. M. Qi , P. F. Greenfield , S. Reid
DOI: 10.1007/BF02215660
关键词:
摘要: A simple protein free medium was formulated and tested in suspension culture using three hybridoma cell lines. The medium, referred to as CDSS (Chemically Defined Serum Substitutes), consisted of the basal DMEM:Ham F12, 1:1, with HEPES (D12H), plus pluronic F68, trace elements, ferric citrate, ascorbic acid, ethanolamine. No or lipid components were added. All lines weaned off serum a commercially available PFHM-II. Data shown here indicated that normally cells took 1-7 weeks wean an additional 2-7 adapt culture. After adaptation able grow well both media main performed better than containing controls. stability for antibody production following freeze/thaw procedures long term subculturing also tested. frozen our (containing 0.75% bovine albumin 10% dimethyl sulfoxide) liquid nitrogen up one year. Cells thawed from these stocks recovered maintain good growth characteristics. One line 12 without loss productivity.
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William Werz, Jutta Maucher, Bettina Reutter, Rolf G. Werner, Wolfgang Berthold, Cryoprotection and cultivation of hybridoma cell lines under serum free condition Animal Cell TechnologyDevelopments, Processes and Products. pp. 149- 151 ,(1992) , 10.1016/B978-0-7506-0421-5.50043-X
Yves-Jacques Schneider, Alain Lavoix, Monoclonal antibody production in semi-continuous serum- and protein-free culture. Effect of glutamine concentration and culture conditions on cell growth and antibody secretion. Journal of Immunological Methods. ,vol. 129, pp. 251- 268 ,(1990) , 10.1016/0022-1759(90)90446-3
O.-W. Merten, J. Litwin, Serum-free medium for fermentor cultures of hybridomas Cytotechnology. ,vol. 5, pp. 69- 82 ,(1991) , 10.1007/BF00365535
Yves-Jacques Schneider, Optimisation of hybridoma cell growth and monoclonal antibody secretion in a chemically defined, serum- and protein-free culture medium. Journal of Immunological Methods. ,vol. 116, pp. 65- 77 ,(1989) , 10.1016/0022-1759(89)90314-1
W. Louis Cleveland, Irene Wood, Bernard F. Erlanger, Routine large-scale production of monoclonal antibodies in a protein-free culture medium Journal of Immunological Methods. ,vol. 56, pp. 221- 234 ,(1983) , 10.1016/0022-1759(83)90414-3
E SHACTER, Serum-free media for bulk culture of hybridoma cells and the preparation of monoclonal antibodies Trends in Biotechnology. ,vol. 7, pp. 248- 253 ,(1989) , 10.1016/0167-7799(89)90016-4
Georg Schmid, Harvey W. Blanch, Charles R. Wilke, Hybridoma growth, metabolism, and product formation in HEPES-buffered medium: II. Effect of pH Biotechnology Letters. ,vol. 12, pp. 633- 638 ,(1990) , 10.1007/BF01088185
Mark C. Glassy, John P. Tharakan, Pao C. Chau, Serum‐free media in hybridoma culture and monoclonal antibody production Biotechnology and Bioengineering. ,vol. 32, pp. 1015- 1028 ,(1988) , 10.1002/BIT.260320809
S.P.C. Cole, E.H. Vreeken, S.E.L. Mirski, B.G. Campling, Growth of human × human hybridomas in protein-free medium supplemented with ethanolamine Journal of Immunological Methods. ,vol. 97, pp. 29- 35 ,(1987) , 10.1016/0022-1759(87)90101-3
G. W. Hiller, A. D. Aeschlimann, D. S. Clark, H. W. Blanch, A kinetic analysis of hybridoma growth and metabolism in continuous suspension culture on serum‐free medium Biotechnology and Bioengineering. ,vol. 38, pp. 733- 741 ,(1991) , 10.1002/BIT.260380707