Serum-free medium for fermentor cultures of hybridomas
作者： O.-W. Merten , J. Litwin
DOI: 10.1007/BF00365535
关键词:
摘要: Hybridomas lend themselves particularly well to large scale cultivation techniques since they grow as single cells in suspension without requiring attachment a substrate. Furthermore, many cell strains have been adapted serum-free (SF) media similar density and antibody production serum containing media. This review will concern itself mainly with the of hybridomas SF-media bioreactors various types ultimate goal producing quantities monoclonal antibodies (mAb).
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sci-hub.se 参考文章(64)
William R. Tolbert, Joseph Feder, Large-Scale Cell Culture Technology Annual Reports on Fermentation Processes. ,vol. 6, pp. 35- 74 ,(1983) , 10.1016/B978-0-12-040306-6.50008-0
H Murakami, Serum-free media used for cultivation of hybridomas. Advances in biotechnological processes. ,vol. 11, pp. 107- 141 ,(1989)
Stephen D. Wolpe, In Vitro Immunization and Growth of Hybridomas in Serum-Free Medium Mammalian Cell Culture. pp. 103- 128 ,(1984) , 10.1007/978-1-4615-9361-4_5
Arye Lazar, Avshalom Mizrahi, Media for cultivation of animal cells: an overview Cytotechnology. ,vol. 1, pp. 199- 214 ,(1988) , 10.1007/BF00145023
Hitoshi Ohmori, Development of a serum-free medium for in vitro immune responses by using β-cyclodextrin: Demonstration of the requirements for polyamines Journal of Immunological Methods. ,vol. 112, pp. 227- 233 ,(1988) , 10.1016/0022-1759(88)90362-6
Hitoshi Ohmori, Itaru Yamamoto, Mechanism of augmentation of the antibody response in vitro by 2-mercaptoethanol in murine lymphocytes Cellular Immunology. ,vol. 79, pp. 173- 185 ,(1983) , 10.1016/0008-8749(83)90060-6
John P. Tharakan, Pao C. Chau, Serum free fed batch production of IgM Biotechnology Letters. ,vol. 8, pp. 457- 462 ,(1986) , 10.1007/BF01025201
T Sato, Y Minamoto, I Yamane, T Kudo, T Tachibana, Spontaneous interferon production and growth of lymphoblastoid cells in serum-free medium Experimental Cell Research. ,vol. 138, pp. 127- 134 ,(1982) , 10.1016/0014-4827(82)90098-2
Huib J.M. van de Donk, Monoclonal antibodies for therapy, prevention and in vivo diagnosis of human disease Journal of Biological Standardization. ,vol. 17, pp. 381- 384 ,(1989) , 10.1016/S0092-1157(89)80010-1
Yves-Jacques Schneider, Optimisation of hybridoma cell growth and monoclonal antibody secretion in a chemically defined, serum- and protein-free culture medium. Journal of Immunological Methods. ,vol. 116, pp. 65- 77 ,(1989) , 10.1016/0022-1759(89)90314-1