Development of a serum-free medium for in vitro immune responses by using β-cyclodextrin: Demonstration of the requirements for polyamines

摘要: Abstract A serum-free medium has been developed which is able to support primary antibody responses by cultured murine lymphocytes. This based on RPMI 1640 that supplemented with β-cyclodextrin, insulin, transferrin, albumin, low density lipoprotein, putrescine and l -alanine as substitutes for fetal calf serum. Omission of anyone these components resulted in a marked decrease responses. By employing the culture conditions, it was clearly demonstrated polyamines such putrescine, spermidine spermine had positive effects development antibody-forming cells. supported response sheep erythrocytes, trinitrophenyl (TNP)-Ficoll or TNP-lipopolysaccharide efficiently 10% serum-containing medium. In addition, lymphocytes proliferated an antigen mitogen equally well two types