Real-time PCR strategy and detection of bacterial agents of lymphadenitis.

摘要: The aim of this study was to compare 16 S rRNA gene amplification and sequencing with a systematic real-time PCR assay screening strategy that includes all common known pathogens recovered from lymph node biopsy specimens. Lymph samples sent our laboratory January 2007 December 2008 were tested in the study. nodes screened for presence any bacteria by targeting also specific Bartonella henselae, mycobacteria, Francisella tularensis, Tropheryma whipplei. By testing 491 nodes, we found sensitivity significantly higher than detection henselae (142 vs 98; p < 10(-4)), tularensis (16 10, mycobacteria (8 versus 3, 10(-4)). None positive Our demonstrates usefulness specificity molecular analysis specimens compared standard sequencing.