Anti-tumor activities and apoptosis-regulated mechanisms of bufalin on the orthotopic transplantation tumor model of human hepatocellular carcinoma in nude mice.

摘要: AIM: To investigate anti-tumor activities and apoptosis-regulated mechanisms of bufalin in the orthotopic transplantation tumor model human hepatocellular carcinoma nude mice. METHODS: BEL-7402 cells were inoculated to form subcutaneous tumors, implanted into liver establish models mice. Seventy-five animals randomized divided five groups (n = 15). Bufalin was injected intraperitoneally three at doses 1.5 mg/kg (BF1), 1 (BF2) 0.5 (BF3) for d 15-24, respectively. The NS group an equal volume saline as above adriamycin ADM a dose 8.0 15. Ten mice each killed 25 survival time calculated. We also observed morphologic alterations myocardium, brain, liver, kidney tissues by pathology electron microscopy, measured apoptotic rate TUNEL staining method, detected expression genes bcl-2 bax immunohistochemical RT-PCR tissues. RESULTS: volumes reduced significantly (35.21 ± 12.51 vs 170.39 25.29; 49.83 11.46 83.99 24.63 25.29, P < 0.01, respectively), times prolonged BF1-2 (31.8 4.2 23.4 2.1 29.4 3.4 2.1, 0.05, necrosis mainly severe or moderate degree BF1-2. No morphological changes tissues. Apoptotic characteristics could be seen positive rates protein 10.0%, 20.0%, 10.0% 20.0%; 90.0%, 80.0%, 40.0% 30.0%, Loss mRNA found density increased progressively with increase RT-PCR. CONCLUSION: has significant no marked toxicity able induce apoptosis transplanted cells. This may mediated via up-regulating gene bax, which involved its mechanism bufalin.