Effect of intracerebroventricular administration of the octadecaneuropeptide on the expression of pro-opiomelanocortin, neuropeptide Y and corticotropin-releasing hormone mRNAs in rat hypothalamus.

摘要: Intracerebroventricular (i.c.v.) administration of the octadecaneuropeptide (diazepam-binding inhibitor [33-50]; ODN) exerts a potent anorexigenic effect in rat. We studied ODN on three neuropeptides involved feeding behaviour: orexigenic peptide neuropeptide Y (NPY) and two peptides, corticotropin-releasing hormone (CRH) pro-opiomelanocortin (POMC)-derived alpha-melanocyte-stimulating hormone. The i.c.v. (0.1 microg/kg 1 microg/kg) mRNA expression peptides male rat hypothalamus was evaluated by semiquantitative situ hybridization. In arcuate nucleus, NPY-expressing neurones were mostly found inner zone close proximity third ventricle. at dose 0.1 induced significant decrease 17.4% NPY expression, while depressing more marked (31.4%) with highest (1 microg/kg). POMC-expressing laterally located nucleus. Administration doses increases 33.5% 27.4% POMC respectively. Labelling obtained CRH cRNA probe essentially distributed throughout medial parvocellular area hypothalamic paraventricular ODN, microg/kg, resulted 17.8% 32.8% decreases present data suggest that might exert its increasing decreasing